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. 2012 Apr 12;8(4):e1002642. doi: 10.1371/journal.pgen.1002642

Figure 3. PCR assay for Mu integration in mutants defective in lysogen recovery.

Figure 3

(A) Control PCR reactions monitoring integration at different time points after infection of wild type BW25113 with Mu::Cm, Mu::Cm(Bam1066) and Mu::Cm(Aam1093) phage. These phages can integrate-replicate, integrate but not replicate, or not integrate, respectively. (B) PCR results for wild type Mu::Cm integration 30 min after infection of mutants in the first three categories shown in Figure 2B. (C) As in (B) but with mutants in the Mu Receptor category. Control reactions with either no template (N), Mu, or genomic DNA templates from uninfected BW25113 host (G) are indicated, along with size markers (M). Reaction products were run on agarose gels and stained with ethidium bromide as described under Methods.