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. 2012 Apr 12;8(4):e1002646. doi: 10.1371/journal.ppat.1002646

Figure 3. GSPL upregulates TLR4 expression in splenic adherent cells.

Figure 3

Splenic adherent cells were infected with LD promastigotes (24 h) at 20∶1 parasite-to-splenic cell ratio followed by treatment with 100 µg/mL of GSPL, β-galactosidase treated GSPL (Gal−GSPL) or GSPL along with 100 ng/mL Polymixin B (GSPL+PB) for 9 h. TLR expressions were also evaluated in unstimulated splenic adherent cells and adherent cells stimulated with GSPL, Zymosan (TLR2) or LPS (TLR4) for 9 h. TLR expression was evaluated by real-time PCR (A) and flow cytometry (B) for individual cover slips. Bar graphs in B show the percent of CD11c+ cells expressing TLR2 or TLR4 in splenic adherent cells. The results are representative of three independent experiments. Error bars represent mean ± SD, n=3. * p<0.0001; paired two-tailed Student's t-test.