Figure 2. Two component system and transporter up-regulation confirmed with qRT-PCR.

A) Expression of ihk, irr, and ABC transporters in intracellular 2h pi and extracellular bacteria determined by qRT-PCR analysis. Normalized mean value of 4 biological replicates shown as fold change in intra- versus extracellular bacteria. B) Expression of selected genes at 2h pi determined by qRT-PCR or microarray. RNA from 3 - 4 biological replicates per method was analyzed. The normalized mean value for each gene is found in the table.