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. 2012 Apr 12;7(4):e32265. doi: 10.1371/journal.pone.0032265

Table 2. Candidate reference genes with respective symbol, accession number, name, primer sequences and efficiency of amplification (E).

Gene Symbol (Accession Number) Gene Name Primer Sequence (5′-3′) E (%)
RPLPO (NM_001002) Ribosomal protein large P0 F: GGATTACACCTTCCCACTTGCT R: GCCACAAAGGCAGATGGATCA 92
RPL27 (NM_000988) Ribosomal protein L27 F: GTGAAAGTGTATAACTACAATCACC R: TCAAACTTGACCTTGGCCT 91
HPRT (NM_000194) Hypoxanthine phosphoribosyl-transferase 1 F: ACCCCACGAAGTGTTGGATA R: AAGCAGATGGCCACAGAACT 91
B2M (NM_004048) Beta-2 microglobulin F: GCTATCCAGCGTACTCCAAAG R: CACACGGCAGGCATACTC 99
ACTB (NM_001101) Beta-actin F: CATCCACGAAACTACCTTCAACTC R: GCAATGATCTTGATCTTCATTGTG 95
18S (NR_003286) 18S ribosomal RNA F: CAGCCACCCGAGATTGAGCA R: TAGTAGCGACGGGCGGTGTG 99
POLR2A (NM_000937) RNA polymerase 2A F: CTTCACGGTGCTGGGCATT R: GTGCGGCTGCTTCCATAA 95
CYCA/PPIA (NM_021130) Peptidylprolyl isomerase A F: ATCCTAGAGGTGGCGGATTT R: CACTCAGGTCTGAGCCACAA 90
GAPDH (NM_002046) Glycerladehyde 3-phosphate dehydrogenase F: ATGTTCGTCATGGGTGTGAA R: GGTGCTAAGCAGTTGGTGGT 97