Table 4. Coefficient of correlation (r), coefficient of variation (CV) and standard deviation (SD) in the Ct values of each candidate gene calculated by the Bestkeeper algorithm for n = 12 and n = 33.

Candidate Genes	N = 12			N = 33
	R	CV	SD	r	CV	SD
POLR2A	0.973	2.04	0.55	0.843	2.13	0.58
CYCA	0.966	0.97	0.22	0.845	1.40	0.32
GAPDH	0.916	1.72	0.40	0.931	1.67	0.39
ACTB	0.912	1.88	0.39	0.878	1.84	0.39
RPL27	0.897	1.58	0.35	0.853	1.42	0.32
B2M	0.858	2.23	0.44	0.902	2.26	0.45
HPRT	0.813	1.47	0.48	0.809	1.34	0.44
18S	0.701	1.96	0.33	0.486	1.84	0.32
RPLPO	0.687	1.97	0.41	0.742	1.82	0.38