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. 2012 Apr 12;7(4):e34325. doi: 10.1371/journal.pone.0034325

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Su et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A representative Western blot of JAM2 protein after luminal epithelial sheets isolated from day 4 of pregnancy were treated in vitro with progesterone for 6 and 12 h, respectively. Progesterone was dissolved in ethanol. Ethanol was used as control. (B) The quantitative analysis in Fig. 3A. (C) Real time RT-PCR of Jam2 mRNA expression in mouse uterus on day 4 of pregnancy after pregnant mice were treated with RU486 (25 mg/kg) twice at 20:00 on day 2 and 08:00 on day 3. (D) A representative Western blot of JAM2 in mouse uterus on day 4 of pregnancy after pregnant mice were treated with RU486 (25 mg/kg) twice at 20:00 on day 2 and 08:00 on day 3. (E) The quantitative analysis in Fig. 3D.