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. 2012 Apr 12;7(4):e34325. doi: 10.1371/journal.pone.0034325

Figure 4. LIF and Stat3 regulation of Jam2 expression.

Figure 4

(A) A representative Western blot of total and phosphorylated Stat3 in mouse uterus after ovariectomized mice were treated with progesterone (P) or a combination of progesterone and RU486. (B) The quantitative data in Fig. 4A. (C) A representative Western blot of total and phosphorylated Stat3 in mouse uterus on day 4 of pregnancy after pregnant mice were treated with RU486 (25 mg/kg) twice at 20:00 on day 2 and 08:00 on day 3. (D) The quantitative data in Fig. 4C. (E) Real time RT-PCR of Jam2 mRNA expression in mouse uterus on day 4 of pseudopregnancy after intrauterine injection of Stat3 phosphorylation inhibitor on day 3. (F) A representative Western blot of JAM2, total Stat3 and phosphorylated Stat3 in mouse uterus on day 4 of pseudopregnancy after intrauterine injection of Stat3 phosphorylation inhibitor on day 3. (G) The quantitative data in Fig. 4F. (H) A representative Western blot of JAM2 and Stat3 in cultured luminal epithelium after luminal epithelial sheets were transfected with c-Stat3 vector (c-Stat3, a continuous activated form of Stat3) and control vector (pcDNA3.1). (I) The quantitative data in Fig. 4H.