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. 2012 Apr 12;7(4):e34425. doi: 10.1371/journal.pone.0034425

Table 1. Oligonucleotides used in this study.

Primers Sequences (5′ to 3′)
VP2-F CGCGGATCC ATGGAGGAGCTGGCTATCCCC
VP2-R GCTCTAGA TTACACGTTCAGAAGCTTCGTAAGC
ORF1 Fw CAGTGAATTCGACGTAACTATAACGGTCCTAAGGTAGCGAATTTTTCCATTGGGCCCCTCCC
ORF1 Rv CGCCTGCAGCTACTAGT TGGAGATGGAGACAGAGGAGG
ORF3 Fw GATC GCATGC CCCGGGGCTAAAAAGCTGCGT
ORF3 Rv GATCAAGCTGACGTAACTATAACGGTCCTAAGGTAGCGAAGGAGCAGCAGGCCCCCATCGA
EF1-ep1 TTTTGCGCACGGTTATGTGGACAAAATACCTGGTTACCCAGGCCGTGCCGGCACGTTAACCGGGCTCGTGAGGCTCCGGTGCCCGTCA
EF1-ep2 TGGTGGCGACCGGTAGCGCTAGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCTCACGACACCTGAAATGGAAGA
IRES Fw CAGT GAATTC GCCCCTCTCCCTCCCCCCCCCCT
IRES Rv ATGC TCTAGA ATTATCATCGTGTTTTTCAAAGGAA
VP5-in Fw ATGCTCTAGATCCAATATGGGCAAAATCATTAAGAGCCTGTCCCGTTGGATGACGACGATAAGTAGGGATAAC
VP5-in Rv ATGC TCTAGA GGG TAA TGC CAG TGT TAC AAC CA
VP5-ep1 CATGTCTTTGGTAACGATGAGATGCTTACGAAGCTTCTGAACGTGTAAGCCCCTCTCCCTCCCCCCCCCCT
VP5-ep2 GCGAGCTCTAGCATTTAGGTGACACTATAGAATAGGGCCCTCTAGATCAGGCATTGCGAAGAAACAATGGG
pRacH-xx-F CCCTCTACGGTTTTCTTCGAGGCCG
pRacH-xx-R CCTAGGCGATGTGTGCAGCCGAGGC