Table 2. Oligonucleotide primers used in this study.
Gene | Primer Name | Sequence (5′-3′) | 5′ Position | Source |
ND2 | L4437 | AAGCTTTCGGGCCCATACC | 3833 | [92] |
ND2r102 | CAGCCTAGGTGGGCGATTG | 4432 | [93] | |
ND4 | ND4I | TGACTACCAAAAGCTCATGTAGAAGC | 10771 | [94] |
ND4R-NZ | CCAAGRGTTTTGGTGCCTAAGACC | 11670 | [40] | |
tRNA-Leu | TACTTTTACTTGGATTTGCACCA | 11691 | [94] | |
Cytochrome b | mtD-25 | CCATCCAACATCTCAGCATGATGAAA | 14202 | [95] |
SkCytBR | TAGGCAAANARRAAGTAYCAYTCTGG | 14940 | [40] | |
Control region | tRNAp-L | GCTAACCCCTCGTCACTAACTCC | [39] | |
CR-Rev-3 | GCACCTGACACTAGTAACGG | This study |
The letters L and H refer to the light and heavy strands. Values in ‘5’ position' refer to the position of the 5′ position in the complete Eumeces egregius mtDNA sequence [96].