Figure 5. Caveolae have little effect on CTxB diffusion at the cell surface.

(A) Subcellular distribution of Alexa546-CTxB, YFP-GL-GPI, YFP-GT46, and DiIC₁₆ in live Cav-1^+/+ and Cav-1^−/− MEF cells. Bar, 10 µm. (B) Diffusion coefficients of Alexa546-CTxB, YFP-GL-GPI, YFP-GT46 and DiIC₁₆ in Cav-1^+/+ and Cav-1^−/− MEF cells (mean ± SD from 22–47 cells). Cells were labeled with 1 µM Alexa546-CTxB. FRAP was performed at 37°C.