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. 2012 Apr 12;7(4):e34658. doi: 10.1371/journal.pone.0034658

Table 1. Summary of results for a series of biochemical analyses on the properties of the LMH monoclonal antibody panel.

Antibody ELISA Positive Flow Cytometry Positive Immunoblot IP Binding Affinity (KD) HGF/SF Antagonista c-MET Agonista Antagonist of HGF/SF- Induced Cell Migrationb Induces Cell Migrationb
LMH 80 Yes Yes Yes, β-chain Yes, c-MET precursor 34 nM No No n.d. n.d.
LMH 81 Yes Yes No Yes, c-MET precursor 12 nM No No n.d. n.d.
LMH 82 Yes Yes Yes, α-chain Yes, c-MET precursor n.d. No No n.d. n.d.
LMH 83 Yes Yes No No 26 nM No No n.d. n.d.
LMH 84 Yes Yes Yes, α-chain Yes 36 nM No No n.d. n.d.
LMH 85 Yes Yes No Yes n.d. No Yes No Yes
LMH 86 Yes Yes Yes, α-chain Yes n.d. No No No No
LMH 87 Yes Yes Yes, α-chain Yes 2.6 nM No No Yes No
LMH 88 Yes Yes Yes, α-chain Yes 76 nM No No Yes No
LMH 89 Yes Yes Yes, α-chain Yes 31 nM No No Yes No

n.d. = not determined; IP = immunoprecipitation; HGF/SF = hepatocyte growth factor/scatter factor.

a

assays performed using A549 lung adenocarcinoma cells.

b

assays performed using SK-OV-3 ovarian carcinoma cells.