Table 1. Summary of results for a series of biochemical analyses on the properties of the LMH monoclonal antibody panel.
Antibody | ELISA Positive | Flow Cytometry Positive | Immunoblot | IP | Binding Affinity (KD) | HGF/SF Antagonista | c-MET Agonista | Antagonist of HGF/SF- Induced Cell Migrationb | Induces Cell Migrationb |
LMH 80 | Yes | Yes | Yes, β-chain | Yes, c-MET precursor | 34 nM | No | No | n.d. | n.d. |
LMH 81 | Yes | Yes | No | Yes, c-MET precursor | 12 nM | No | No | n.d. | n.d. |
LMH 82 | Yes | Yes | Yes, α-chain | Yes, c-MET precursor | n.d. | No | No | n.d. | n.d. |
LMH 83 | Yes | Yes | No | No | 26 nM | No | No | n.d. | n.d. |
LMH 84 | Yes | Yes | Yes, α-chain | Yes | 36 nM | No | No | n.d. | n.d. |
LMH 85 | Yes | Yes | No | Yes | n.d. | No | Yes | No | Yes |
LMH 86 | Yes | Yes | Yes, α-chain | Yes | n.d. | No | No | No | No |
LMH 87 | Yes | Yes | Yes, α-chain | Yes | 2.6 nM | No | No | Yes | No |
LMH 88 | Yes | Yes | Yes, α-chain | Yes | 76 nM | No | No | Yes | No |
LMH 89 | Yes | Yes | Yes, α-chain | Yes | 31 nM | No | No | Yes | No |
n.d. = not determined; IP = immunoprecipitation; HGF/SF = hepatocyte growth factor/scatter factor.
assays performed using A549 lung adenocarcinoma cells.
assays performed using SK-OV-3 ovarian carcinoma cells.