Figure 2.
Overall topology of M8VL and M8VLSL55P bound to DIR. (A,B) M8VL binds DIR (only one DIR conformation is shown for clarity) sandwiched between two identical VL domains (blue and light blue). The CDR1, CDR2, and CDR3 loops are colored orange, pink, and green, respectively, and are labeled in panel A. The view in (B) is rotated 90° about a horizontal axis. (C,D) M8VLSL55P binds DIR in an almost identical way as M8VL. The SL55P mutation is the only sequence difference between the two structures. The structure figures were generated with Molscript (59) and Bobscript (60).