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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Biochemistry. 2012 Mar 14;51(12):2471–2485. doi: 10.1021/bi201422g

Table 2.

Results of altering glycine-serine rich linker length in tandem homodimers created from the M8VL.

Clone (G4S)
repeats in
linker		 Fluorescence
Enhancement
(YSD)* 		Soluble KD ϕ f
dM8VL(G4S)3 3 1.0 <0.1† nM 0.38
dM8VL (G4S)4 4 2.0 <0.1† nM 0.64
dM8VLSL55P(G4S)6 6 2.0 <0.1† nM 0.55
dM8VLSL55P(G4S)3 3 3.4 <0.1† nM 0.14
*

Fluorescence enhancement is calculated as DIR fluorescence signal normalized for cell surface expression for each individual clone, divided by the equivalent value for the M8VL. The concentration of dM8VL (G4S)3 was 3 nM, dM8VL(G4S)4 was 3 nM, dM8VL(G4S)6 was 3nM, and dM8VLSL55P was 12 nM.

†

Data were equally well fit to Kd values less than 0.1 nM.