Figure 2.

Summary of the procedure to evaluate inhibitor binding selectivity to TTR versus other plasma proteins. Human blood plasma is incubated with a small molecule inhibitor (black bars) for 24 h. The TTR tetramer (squares) and bound small molecules are precipitated with antibody-conjugated Sepharose (circle). The Sepharose is washed to remove unbound proteins (oval and diamond) and small molecules (triangle). TTR with (shown here) or without ligands bound is eluted at high pH. The supernatant is loaded onto an HPLC column for analysis and quantification.