Figure 4.
Lytic activity of purified NK cells in short-term ethanol-consuming mice is comparable to water controls on a per cell basis. After acclimation, mice were maintained on 20% ethanol for one week. NK cells were purified from spleen using positive selection. The cells were cultured overnight in the presence of 200 units/ml IL-2 and lytic activity was tested by chromium release using YAC-1 target cells. The purified NK cells from ethanol consuming mice were comparable to water controls in augmenting their lytic activity in response to IL-2