Figure 3.

Exogenous NGAL exacerbates NTN. 129/SvJ mice received nephrotoxic serum, and in addition received a single injection of 250 μg of NGAL (“NTN+NGAL”; n=10) or PBS (“NTN alone”; n=10) intravenously on d6. An additional control group of mice was immunized with rabbit IgG as above but received an injection of PBS on d5, and an injection of 250 μg NGAL on d6 (“NGAL alone”; n=10). (A) Proteinuria on d7. (B) Mice were sacrificed on d8, and the kidney paraffin sections were stained for analysis of histopathology. Mice receiving NGAL without concurrent induction of NTN all scored 0 for each of the kidney sub-scores, and therefore these are not evident in this graph. Since all mice in the NTN+NGAL group had identical scores for each subcategory, the error bar equals 0 for this group. (C) NTN was induced in an independent cohort as above, with mice treated with NTN+NGAL (n=10), NTN alone (n=10), and NGAL alone (n=10). Starting from d5, survival was monitored daily. Mice appearing moribund were sacrificed without knowledge of the treatment assignment. The p values indicated refer to the comparison between NTN+NGAL and NTN alone groups, **:p<0.01; ***: p<0.001.