Fig. 2.

Dual luciferase reporter assays. The ratios of Firefly luciferase activity (signal S) to Renilla luciferase activity (control C) are displayed using box and whisker plots. Three subcloned plasmids were prepared, and each subcloned plasmid was tested in triplicate and S/C values were averaged. The experiments were carried out twice independently. As a result, six independent S/C values were obtained for each haplotype. ATC haplotype showed significantly higher transcriptional activity than CCC haplotype and ATT haplotype (*, **P = 0.037, respectively) by Mann–Whitney U test. No significant difference was observed between CCC and ATT haplotypes (data not shown)