Fig. 1.

Comparison of relative expression levels of major alternatively spliced mRNA transcripts of GH2 (A–C), CSH1 (D and E), and CSH2 (F and G) in placentas from uncomplicated pregnancies (control, n = 17) and complicated with pre-eclampsia (PE, n = 17). The corresponding transcript representing the presented data is shown on the top of each panel. Exons are shown as boxes and introns as lines. The coding region is denoted by black/grey-filled area. An asterisk (∗) denotes the 4 bp deletion in exon 4 of GH2-3. Relative expression of each transcript is given as ratio to the reference gene GAPDH. The boxes represent the 25th and 75th percentiles. The median is denoted as the line that bisects the boxes. The whiskers are lines extending from each end of the box covering the extent of the data on 1.5× interquartile range. Circles represent the outlier values. Plotted values are presented without covariate adjustment. Statistical differences between study groups were assessed by ANCOVA using Bonferroni correction. Statistical tests were adjusted for newborns birth-weight, birth length, and abdominal circumference. Bonferroni corrected P-values reflecting significant differences (P < 0.05) are shown.