Figure 5.

Regulation of COX-1-IR in vascular cell types. Mean ± SEM density (cell number per square millimeter vascular area) of cells identified on morphological grounds as perivascular (black bars) or endothelial cells (gray) as a function of treatment status. Under control (Con) conditions, COX-1 is detected only in perivascular cells, and their number is not significantly affected at 1 h after an LPS challenge. LPS does, however, result in enzyme induction in endothelia and a highly reliable increase in total vascular COX-1-positive cell density. Perivascular cell depletion after intracerebroventricular clodronate liposome (Lip) treatment results in enhanced endothelial COX-1 expression under both control and LPS challenge conditions. n = 3 per group. *p < 0.001 versus saline-injected counterparts. ^†p < 0.001 versus LPS-injected control animals.