Figure 7.

Fine structure of COX-1-immunoreactive vascular and parenchymal cells. Immunoelectron micrographs showing features of COX-1-immunoreactive labeling of perivascular (top), microglia (middle), and endothelial (bottom) cells. In PVCs, COX-1-IR displays a continuous association with the nuclear membrane (nm, arrows in A) and is also observed in portions of the endoplasmic reticulum (er) near the nucleus (n; see B). In microglial cells, identified on the basis of size, and relatively scant and electron-dense cytoplasm, reaction product in microglia was densely distributed throughout the cytoplasm (C) but, in a few cases, was restricted to the cytoplasmic membrane (D, arrowheads). COX-1-immunoreactive labeling of EC patchy accumulations of reaction product associated with the nuclear membrane (E, F). In addition, COX-1-immunoreactive labeling was occasionally observed in lysosomes or multivesicular bodies (MVB in G). gl, Glia limitans; SM, smooth muscle cell. Scale bars, 1 μm.