Fig. 2.
Transient expression of Art v 1 in leaves of transformed tobacco plants. Tobacco (Nicotiana tabacum) leaves were transiently transformed by pressure infiltration with Agrobacterium tumefaciens cultures at the lower leaf site. a Art v 1-specific glycosylation and the Art v 1 protein were detected in leaf cell lysates after 2 days in transformed leafs (Art v 1). Water-infiltrated leaves (WT*IF) did not show any signals. A pollen exudate (PE) from Artemisia vulgaris served as a positive control. b Infiltrates were harvested from leaves transformed with Agrobacteria containing the signal peptide of Art v 1 fused to GFP (SPArtv1::GFP) to verify targeting to the apoplast. GFP-specific signals at 27 kDa were observed at 2 and 5 days after transformation (arrow). 20 μg protein per lane, antibodies as indicated (see “Materials and methods”)