Fig. 3.

Stable expression of Art v 1 in leaves of transformed tobacco plants. Transformed tobacco plants (Nicotiana tabacum) were generated by co-cultivation of leaf discs with Agrobacterium tumefaciens containing the pBI121_Artv1 plasmid and regeneration in the presence of kanamycin. Upper panel PCR with Art v 1-specific primer resulted in a specific product of 620 bp in the control plasmid (C, pBI121_Atrv1) and in plant nos. 1–5. An unspecific background signal of 500 bp is detectable in transformants but also in PCR experiments using DNA from wild-type tobacco. Lower panel Immunodetection of recombinant Art v 1 in leaf lysates of transformed tobacco plants 1–5. A typical double protein band of 20–25 kDa was detected by an anti Art v 1 antibody in the transformants 1–5. Purified, native Art v1 (nArtv1) and a mugwort pollen exudate (PE) served as positive controls. No protein signals were observed in the leaf lysate of wild-type plants (WT). 25 μg protein per lane. Plant no. 5 (in red) was used for further analysis