Fig. 4.

Recognition of plant recombinant Art v 1 by allergenic patient sera. Cell lysate proteins prepared from transformed plant no. 5 of Fig. 3 (transformed, pBI121_Artv1) were separated by SDS-PAGE and plant recombinant (plant rArtv1), bacteria recombinant (E.coli rArtv1) and native Art v 1 (natArtv1), respectively, were detected by Art v 1-specific IgEs from various patient sera as indicated by patient numbers. No Art v 1-specific signals were detectable in wild type leaf lysates or with a pool of non-atopic human sera (NHS). 25 μg protein per lane. BC buffer control