Figure 1.

No evidence of the 55-kDa band was found in serum samples of the subgroup of 30 patients who were tested for, indicating no presence of auto-antibodies against osteoprotegerin. A: Western blotting (representative serum samples from a series of 30 celiac disease patients) showing the absence of antibodies against osteoprotegerin (OPG) after immunoprecipitation. Positive control indicates a 55 kDa band (arrow, lane 2) as the presence of antibodies against OPG. Negative control is shown in the lane 1; B: Western blotting confirms the equal loading of the gel by means of the addition of staining for immunoglobulins. Molecular-weight markers are shown in lane M.