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. Author manuscript; available in PMC: 2012 Apr 13.
Published in final edited form as: Ann N Y Acad Sci. 2009 May;1165:169–174. doi: 10.1111/j.1749-6632.2009.04060.x

Figure 5.

Figure 5

Point mutations within the SH3 binding region of EspF prevent interaction with SNX9. Short peptides homologous to the SH3 binding region of EspF were synthesized such that sequential residues were point-mutated to aspartate. These mutated peptides were affixed to nitrocellulose along with a wild-type peptide, overlaid with radio-labeled SNX9 and radioactive signal due to bound SNX9 visualized by autoradiogram. These studies define specific amino acids that are required for EspF-SNX9 interaction.