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Complement anaphylatoxin C3a serum concentrations in C57BL/6 wild-type (n=22) and Rag1−/− mice (n=23) at 4 h, 72 h, and 7 days after closed head injury. C3a levels were determined by a mouse-specific ELISA and normalized by total protein concentration, as described in the methods section. Data are shown as medians±standard deviation (*p<0.05 for wild-type versus Rag1−/− mice at all time points; ELISA, enzyme-linked immunosorbent assay).
