FIG. 4.

Representative Western blot analysis of brain homogenates from injured (left) and uninjured (right) hemispheres of C57BL/6 wild-type (WT) and Rag1^−/− mice at 4 h after sham surgery or closed head injury. Equal concentrations of protein (50 μg per lane) were loaded on SDS-Page membranes, and consistent loading was confirmed by β-actin control blotting. Mouse-specific primary antibodies against Fas, FasL, Bax, and Bcl-2, were used on nitrocellulose membranes and visualized by a colorimetric assay using alkaline phosphatase, as described in the methods section.