FIGURE 1.

Subcellular localization of protein constructs upon transfection and FRAP of NES-GFP. a, cellular localization of NES-GFP, NLS-mCherry, and GFP analyzed by confocal imaging. Scale bars, 10 μm. b, FRAP analysis of NES-GFP. The plot reports the time-recovery of nuclear (blue circles) and cytoplasmic (red squares) fluorescence of NES-GFP following the photobleaching step; the continuous lines represent monoexponential fitting. Fluorescence confocal images of a cell undergoing FRAP are reported in the inset together with the acquisition time.