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. 2011 Sep 5;31(15):1988–1994. doi: 10.1038/onc.2011.387

Figure 1.

Figure 1

EZH2 depletion leads to increased KLF2 mRNA and protein levels. Expression of KLF2 and EZH2 determined by qRT–PCR (a) and (b) immunoblot in five cancer cell lines (U2OS, MDA-MB-231, MCF-7, PC3 and LNCaP cells) following transfection with oligo-type short interfering RNA (siRNA) against EZH2 or scrambled siRNA for 72 h as a transient model. (c) Immunoblot of EZH2 and KLF2 in stable EZH2 knockdown clones. U2OS cells are transfected with shRNA constructs targeting EZH2 or control vector and undergo selection with puromycin. P-values obtained from Student's t-test.