Skip to main content
. Author manuscript; available in PMC: 2012 Apr 13.
Published in final edited form as: Dev Cell. 2010 Apr 20;18(4):533–543. doi: 10.1016/j.devcel.2010.02.013

Figure 2. Plk1 activity is required for mitotic entry but not for CyclinB1-Cdk1 activation per se.

Figure 2

A. The FRET signal was recorded (1 image/2min) during mitotic entry in HeLa cells expressing the CyclinB1-Cdk1 activity sensor before (left panel) and after (right panel) addition of Plk1 inhibitor BI2536 (100 nM) to the same dish. Average curve of 3 cells is displayed for each condition. B. RPE cells were synchronized in G2 phase by release from serum starvation and recorded at 1 image/2 min by DIC microscopy. The peak of mitotic entry is indicated by vertical dashed lines (~27-32h after serum re-addition). The addition of the Plk1 inhibitor BI2536 100 nM rapidly induces a strong delay in mitotic entry.