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. Author manuscript; available in PMC: 2012 Apr 13.
Published in final edited form as: Dev Cell. 2010 Apr 20;18(4):533–543. doi: 10.1016/j.devcel.2010.02.013

Figure 7. CyclinB1 degradation is triggered by low levels of CyclinB1-Cdk1 activity and is independent of NEBD.

Figure 7

HeLa cells co-expressing the CyclinB1-Cdk1 biosensor and a CyclinB1-Cdk1AF-mCherry fusion protein. Three cells entering M phase-like states without cytokinesis are displayed. Low CyclinB1-Cdk1 activity, visualised by a weak increase in FRET activity, is sufficient to trigger nuclear import of CyclinB1-Cdk1AF and its subsequent degradation in the absence of NEDB. The onset of CyclinB1-Cdk1AF degradation is indicated on the pseudo-colour (rainbow LUT) images. For each cell, the quantification of the FRET activity, the level of CyclinB1-Cdk1AF-mCherry and its nuclear accumulation are shown.