Fig. 6.

The chimera that has the EAS sequence between Cys7 and Cys8 forms ThT-positive rodlets. (A) Sequences of NC2, EAS, EChi2, and NChi2. (B) Agitation of WT EAS, NC2, NChi2, and EChi2 at pH 2.5 and 45 °C results in formation of ThT-positive rodlets by WT EAS (solid black line) and NChi2 (dotted black line) but not by the class II hydrophobin NC2 nor the EChi2 chimera (solid gray lines). Variation in the length of the lag phase was observed among replicate samples of NChi2. Three replicates each are presented. (C) TEM of the NChi2 chimera sample produced in the ThT assembly assay shows that the assemblies formed by this chimera display the characteristic hydrophobin rodlet morphology.