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Sequence of stretching events at extensions smaller than 30 Å. Unfolding proceeds through the following sequence of events: (a) equilibrated structure, (b) prestretched equilibrated or twisted state, (c) intermediate or aligned state, and (d) rupture of the cluster of hydrogen bonds between the F- and G-strands. The corresponding time points are indicated in the extension time curve shown below for FN-III7 unfolded at a constant force of 800 pN. Structures were made by using vmd (29).
