Fig. 4.
C4F6 does not robustly detect hydrophobic SOD1. Spinal cord from 135-d hSOD1G93A mouse separated by hydrophobic interaction chromatography and visualized through immunoblot following denaturing SDS/PAGE. C4F6 (Upper) detects the input (lane 1) and flow through not caught on the column (2, 3) but only weakly detects hydrophobic protein eluted off the column (4–6). A pan-SOD1 antibody (Lower) demonstrates that hydrophobic SOD1 is present in the eluate. Blot is representative of duplicate experiments.