Time course of in situ of PK2 hybridization mRNA in a model of an in vivo stroke. (A) Cresyl-violet staining of brains subjected to moderate (striatum infarct only) and severe (striatum and cortex) stroke in the tMCAO model. (B) In situ hybridization showed that PK2 expression is induced biphasically in the brains subjected to severe stroke: A small peak at 3 h is followed by a larger peak at 24 h. (C) Representative autoradiographic images of PK2 expression 1, 3, 6, 12, 24, and 48 h after tMCAO. Error bars represent SEM. n = 5–6 per time point.