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. 2012 Mar 19;109(14):5529–5534. doi: 10.1073/pnas.1203094109

Fig. 5.

Fig. 5.

siRNA production and AGL expression are dependent on RNA polymerase IV (NRPD1A) in endosperm and a model for endosperm development in interploidy crosses. (A) qRT-PCR analysis (relative expression levels, REL) of NRPD1a expression in endosperm of interploidy crosses. (B–D) qRT-PCR analyses of AGL62 (B), AGL91 (C), and AGL40 (D) expression (n = 3 biological replicates). (E) Small RNA blot analysis of p4-siRNA (siR02), AGL40-siRNA, and AGL91-siRNA in endosperm of interploidy crosses (n = 2 biological replicates). (Left) AGL91-siRNAs were present in seeds but not in siliques. miR166 was used as a control. (F) Model for the role of maternal siRNA-mediated AGL expression in endosperm and seed development (see text for explanation). Multiple dots and an elongated black rod in each diagram represent the endosperm and embryo cells, respectively.