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. 2012 Mar 15;109(14):5399–5404. doi: 10.1073/pnas.1120606109

Fig. 2.

Fig. 2.

The lock-and-key motif is necessary for TIGIT/PVR complex formation. (A) BLI sensograms of the binding of PVR-Fc WT to TIGIT-Fc WT or TIGIT-Fc single point mutants immobilized on anti-human Fc biosensors; data are representative of two experiments. (B) BLI sensograms of the binding of PVR-Fc WT or PVR-Fc single point mutants to TIGIT-Fc WT immobilized on anti-human Fc biosensors; data are representative of two experiments. (C) Flow cytometry analyses of TIGIT-Fc WT or TIGIT-Fc single point mutants binding to BJAB cells stably expressing PVR. The gray-shaded histogram represents binding to nontransfected BJAB cells; the black-shaded histogram represents binding of WT TIGIT-Fc; the unfilled histogram represents the binding of the indicated point mutant. (D) Flow cytometry analysis of PVR-Fc WT or PVR-Fc point mutants binding to BJAB cells stably expressing TIGIT; data are representative of two experiments. The gray-shaded histogram represents binding to nontransfected BJAB cells; the black-shaded histogram represents binding of WT PVR-Fc; the unfilled histogram represents binding of the indicated point mutant.