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Expression of Ptch1/Gli2 in squamous dysplasia of rat esophagus Expression of Ptch1/Gli2 was detected by immunohistochemistry using specific antibodies to Ptch1 and Gli2 (see methods). Please note that the staining (Brown) is mainly in the cytoplasm of the epithelium with some in the nucleus, which was also reported previously for interactions with cyclin B1 [44]. Gli2 was mostly detected in the nucleus.
