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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Cancer Res. 2011 Dec 13;72(3):736–746. doi: 10.1158/0008-5472.CAN-11-2584

Figure 2.

Figure 2

Characterization of the Taspase1 cleavage site consensus motif. A, Alignment of known Taspase1 cleavage sites (CS). h, m, z, and d denote human, mouse, zebra fish, and Drosophila, respectively. B, Alanine scanning mutagenesis of the CS2 region of human MLL. The indicated p45MLLCS2 (aa 2,500 to 2,800) cleavage reporters were generated by single alanine substitution of individual amino acid across P7 to P5′ of CS2, where the CS1 was invariably mutated to enable assessment of a single cleavage site. The individual IVTT, 35S-methionine labeled, p45MLLCS2 reporters were incubated with 15 ng of rTaspase1 (recombinant Taspase1) for 30 minutes at 30°C, resolved by SDS-PAGE, and monitored by autoradiography.