Figure 3.
CPTEG:CPH nanoparticle chemistries released biologically active and antigenic PA. A) Residual antigenicity and B) residual activity of PA after two days of release from polyanhydride nanoparticles. The direct ELISA was performed with 0.5 μg/mL PArel from the nanoparticles. The biological assay was performed by incubating 6.4 μg/mL PArel from polyanhydride nanoparticles with 0.3 μg/mL LF with RAW cells for 6 h. Error bars represent standard deviation of 3 replicates. Treatments with different letters are significantly different from one another at p < 0.05.