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SDS–PAGE gel of McpS-LBD incubated with malic acid. Crystals were resuspended in 20 µl reservoir buffer and subjected to several rounds of washing by centrifugation. Lane 0 contains the protein used for the crystallization experiments. Lanes 1–6 contain the supernatant from subsequent washing steps. After the final wash, crystals were resuspended in reservoir buffer followed by addition of 10 µl 1× loading buffer prior to analysis by SDS–PAGE (lane 7).
