Table 1.
PHA production and relative monomer composition by P.putida KT2442 in continuous culture at D = 0.1 h-1
| Limitation | (C0/N0)* | Y X/C | Y X/N | (C0/N0)b | Residual carbona | Residual nitrogena | CDWa | PHA contenta | Monomer composition (mol %) |
||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Borders | |||||||||||
| (mol mol-1) | (g g-1) | (g g-1) | (mol mol-1) | (g) | (g) | (g l-1) | (% CDW) | C6 | C8 | C10 | |
| Carbon | 5.83 | 1.11 | 9.80 | 10.40 | N.D. | 0.112 ± 0.01 | 1.46 ± 0.04 | 25.78 ± 0.97 | 3.3 | 53.4 | 43.3 |
| Carbon-Nitrogen | 16.56 | 1.16 | 16.67 | N.D. | N.D. | 4.35 ± 0.13 | 61.94 ± 4.95 | 4.8 | 42.8 | 52.4 | |
| Nitrogen | 26.97 | 1.01 | 17.74 | 20.50 | 1.53 ± 0.04 | N.D. | 4.63 ± 0.14 | 80.58 ± 0.24 | 4.3 | 40.2 | 55.5 |
a Standard deviation (±) from at least three independent experiments
*C0/N0: Initial feeding ratio of carbon-nitrogen
N.D., Not detectable
b According to the procedure described by Egly and Quayle (1986)