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. 2012 Mar 27;31(1):27. doi: 10.1186/1756-9966-31-27

Figure 3.

Figure 3

Down-regulation of WT1 by siRNA could not increase the expression of miR-15a/16-1 in leukemic cells. (A and B) K562 and HL-60 cells were transfected with 50 nM siRNA-WT1, 50 nM N.C or neither of the above for 24 and 48 hours, then the relative mRNA expression of WT1 and the corresponding WT1 protein were respectively measured by quantitative real-time PCR and Western blotting. GAPDH as loading control. (C and D) The relative expressions of miR-15a and miR-16-1 were measured by qRT-PCR after K562 and HL-60 cells were transfected with 50 nM siRNA-WT1, 50 nM N.C or neither of the above for 24 and 48 hours. * and &P < 0.01 versus negative control (N.C).