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. 2012 Mar 27;31(1):27. doi: 10.1186/1756-9966-31-27

Figure 4.

Figure 4

Anti-miR-15a/16-1 oligonucleotides (AMO) partly reversed the downregulation of WT1 induced by curcumin in K562 and HL-60 cells. (A and B) The relative expressions of miR-15a/16-1 were measured by qRT-PCR after K562 and HL-60 cells were transfected with 50 nM of anti-miR-15a/16-1 oligonucleotides for 48 hours. * and &P < 0.01 versus negative control (SCR). (C and D) 20 uM curcumin treated-K562 and 10 uM curcumin treated- HL-60 cells were transfected with 50 nM of anti-miR-15a/16-1 oligonucleotides for 48 hours, then the protein levels of WT1 were measured by Western blotting. GAPDH as loading control. (E and F) 20 uM curcumin treated-K562 and 10 uM curcumin treated- HL-60 cells were transfected with 50 nM of anti-miR-15a/16-1 oligonucleotides for 24, 48, and 72 hours, then cell proliferation was measured by CCK-8 assay. # and $ represent less than 0.05 of p-values, compared respectively with pure curcumin treatment alone at the same time.