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. Author manuscript; available in PMC: 2012 Apr 16.
Published in final edited form as: Cell Cycle. 2009 Aug 8;8(15):2451–2462. doi: 10.4161/cc.8.15.9219

Figure 3. Kin1 down-regulation promotes mis-specification of the division site.

Figure 3

nmt81-controlled kin1 cells were cultured in the absence (ON) and in the presence (OFF) of thiamine for 16h at 30°C to repress Kin1 expression. (A) cells were stained with methyl blue in the Kin1 ON and Kin1 OFF conditions. (B) Percentage of cells showing a short/long cell length ratio of the indicated values. The short and long distances separating the septum to cell ends were measured in septated cells of the Kin1 ON and Kin1 OFF conditions and a short/long distance ratio was calculated. Ratios <0.85 and >0.85 refer to asymmetric and symmetric septa, respectively. Bars: 5 μm.