Figure 6. DIM induces apoptosis in CEM cells as detected by TUNEL.

The In situ cell death detection kit (TUNEL) was applied to fixed CEM cells treated with 0 to 15 µM DIM for 48 hr. (A) Fluorescence images of control (0 µM) and DIM-treated (15 µM) cells were taken at 20× magnification following TUNEL labeling in mounting medium with DAPI. (B) Flow cytometry was used to identify and quantify cells with no, low (open bar) or high (solid bar) intensity staining. **, p<0.01 or ***, p<0.001 as determined by one-way ANOVA with Dunnett's post-hoc test comparisons for significant effects of DIM treatments within each intensity category as compared to vehicle control (0 µM DIM, 0.1% DMSO).