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. 2012 Apr 13;7(4):e34883. doi: 10.1371/journal.pone.0034883

Figure 1. P. aeruginosa PAO1 response to k-opioid during growth in nutrient poor medium.

Figure 1

(A) Effect of 200 µM U-50,488 on PAO1 cell density, n = 3. (B) Pyoverdin production normalized to cell density, n = 3, *p<0.01. (C) Pyocyanin production normalized to cell density, n = 3, *p<0.05. (D–D″) Transmission electron microscopy images of PAO1 cells grown in (D) 0.1xTY, and (D′, D″) grown in 0.1xTY supplemented with 200 µM U-50,488. Flagella (D′) and vesicles (D″) are shown by arrows. (E) Kaplan-Meier survival curves in C. elegans feeding in P. aeruginosa PAO1 grown in poor nutrient medium vs poor nutrient medium containing 50 µM U-50,488. Cell cultures were collected at 7 hrs. Cumulative survival is represented as n = 7/plate, 3 plates/experiment, p<0.01.