Figure 4. Virulence expression in response to k-opioid at high and low phosphate concentrations.
(A) MvfR expression measured by β-galactosidase activity in PAO1/mvfR'-lacZ. (B) pyocyanin production normalized to cell density. (C) pyoverdin production normalized to cell density. (D) Mortality in C. elegans incubated with PAO1 grown in 0.1xTY media plus 200 µM U-50,488 with and without supplementation with Pi. (E–I) Heat maps of gene expression related to (E) low phosphate signaling/acquisition, (F) low iron signaling/acquisition, (G) quorum sensing, (H) multi-drug resistance, and (I) stress response.