Table 1. Summary of the MS analysis for identification of the rat proteins in the Coomassie Blue-stained bands.
| Band # (size) | Accession Number* | Protein identified | (Peptides) | % Seq. |
| Sample 1 | ||||
| Band 1 (400 kDa): | NP_112257.1 | MAP1A | (4) | 1.7 |
| NP_062172.1 | Clathrin heavy chain | (3) | 2.1 | |
| Band 2 (350 kDa): | No data | |||
| Band 3 (145 kDa): | NP_113916.1 | SNAP 91/AP180 | (5) | 4.7 |
| Band 4 (110 kDa): | NP_001100981.1 | AP2 α1-adaptin | (6) | 6.6 |
| NP_058973.1 | AP1 β1-adaptin | (5) | 6.6 | |
| NP_542150.1 | AP2 β1-adaptin | (4) | 5.2 | |
| NP_062172.1 | Clathrin heavy chain | (2) | 1.4 | |
| NP_001101419.1 | DNAJ Hsp40 homolog | (2) | 2.4 | |
| Band 5 (30 kDa): | No data | |||
| Sample 2 | ||||
| Band 1 (400 kDa): | NP_112257.1 | MAP1A | (13) | 5.5 |
| NP_062172.1 | Clathrin heavy chain | (8) | 4.5 | |
| Band 2 (350 kDa): | NP_062172.1 | Clathrin heavy chain | (3) | 1.8 |
| NP_112257.1 | MAP1A | (3) | 1.4 | |
| NP_037198.1 | MAP2 | (2) | 1.4 | |
| Band 3 (145 kDa): | NP_062172.1 | Clathrin heavy chain | (24) | 12.0 |
| NP_113916.1 | SNAP 91/AP180 | (9) | 7.4 | |
| NP_476459.1 | Contactin 1 precursor | (5) | 4.4 | |
| Band 4 (110 kDa): | NP_112270.2 | AP2 α2-adaptin | (9) | 8.9 |
| NP_542150.1 | AP2 β1-adaptin | (6) | 7.0 | |
| NP_001100981.1 | AP2 α1-adaptin | (4) | 3.2 | |
| Band 5 (30 kDa): | NP_062039.1 | Dyrk1A | (5) | 14.1 |
| GT26_SCHJA | Synaptogyrin 1 | (2) | 10.3 | |
| Glutathione S transferase† | (14) | 36.7 | ||
| Sample 3 | ||||
| Band 1 (400 kDa): | NP_112257.1 | MAP1A | (4) | 1.6 |
| Band 2 (350 kDa): | NP_062172.1 | Clathrin heavy chain | (3) | 1.8 |
| Band 3 (145 kDa): | NP_113916.1 | SNAP 91/AP180 | (8) | 6.6 |
| NP_062172.1 | Clathrin heavy chain | (7) | 4.0 | |
| NP_112399.1 | Tripeptidyl peptidase II | (5) | 4.1 |
*
All database searches were performed using the rat-specific RefSeq database (version 9-21-2009).
†
The identifications of glutathione S transferase (GST) and the GST-Dyrk1A fusion protein were made from searches of the SwissProt database (version circa 2005) and a version of that database containing several GST fusion protein sequences, including GST-Dyrk1A.