Figure 4. ADRP, a marker of lipid droplets, decorates the intracellular lipid structures induced by Chs-LDL.

RAW cells were incubated for 48 h with Ac-LDL (A-A^III) or Chs-LDL (B-B^III). Panels (A) and (B) are confocal single-slice images and show the neutral lipids and the ADRP distribution. Neutral lipid-rich structures, in green, were stained with Bodipy 493/503. ADRP, in red, was visualized by immuno-staining with polyclonal antibodies as described under Methods. Panels (A) and (B) are merged images. In panels (A^I) and (B^I) the regions outlined with the rectangles in panels (A) and (B), respectively, are enlarged. Panels (A^II) and (B^II) show Bodipy staining 493/503, panels (A^III) and (B^III) show the distribution of ADRP. Bars, 10 µm.